8:00 am Morning Coffee & Registration

Bridging the Gap to Purification Prevention: Advancing Upstream Process Execution to Ensure RNA Purity from the Outset & Eliminate the Need for Purification

9:00 am Reviewing the Relationship between DNA Starting Materials & IVT Fidelity to Build RNA Purity From the Beginning

• Diana M Posadas Director - Strategic Research, GreenLight BioSciences

9:30 am Roundtable Discussion: Defining Upstream Material Specs in Line with Analytics to Minimize Impurity Risk and Strengthen Process Control

• Zhinan Xia CSO, Abimmune

10:30 am A Roadmap to Better Designer Polymerase Enzymes: Decoding IVT Reaction Kinetics to Define Optimal Mutant Polymerases That Minimize DSRNA Formation & Preserve Yield

• Nick Skizim Senior Director, Analytical Sciences, GreenLight BioSciences

11:00 am Morning Break

Evaluating Novel Purification Methods to Reduce Number of Downstream Steps & Build More Robust & Reproducible Purification Processes at Scale

12:00 pm Optimizing Next-Gen Membrane-Based affinity Purification to Enhance Resolution & Reduce Process Complexity

• Riddhi Banik Researcher, Georges Belfort Lab, Rensselaer Polytechnic Institute

12:30 pm Roundtable Discussion: Selecting the Right Capture Method: Comparing Resin Based, HIC & Affinity Techniques Across Modalities

1:30 pm Lunch Break

Advancing dsRNA Removal Strategies to Improve Purity, Reduce Immunogenicity, & Meet Regulatory Expectations

2:30 pm Implementing Layered Control Strategies to Mitigate dsRNA Production & Downstream Processing

• Linnea Jansson-Fritzberg Scientist II, Editas Medicine

3:00 pm Harnessing Raman Spectroscopy vs IR Spectroscopy to Improve dsRNA Detection & Strengthen In-Process Control Strategies

• Guohua Yin Associate Professor, Qilu Insitute

3:30 pm Unlocking Next-Gen Sequencing for In-Process Detection of dsRNA Impurities

• Darren Begley Associate Director, Process Analytics (US), GlaxoSmithKline

4:00 pm End of Pre-Conference Purification Focus Day