Junta Shimada
Researcher, Downstream Processing CMC Astellas
Seminars
Tuesday 29th September 2026
Designing Linear DNA Downstream Processes Beyond UF/DF Optimization: Mechanistic Insights and Process Redesign to Support Integrated mRNA Manufacturing
9:00 am
- Investigating intrinsic yield loss mechanisms in linear DNA UF/DF that are not universally resolved by operational optimization alone
- Demonstrating plasmid-specific limitations of UF/DF through mechanistic analysis of flow-induced elongation and protein–DNA interactions
- Redesigning downstream processes by decoupling protein removal and buffer exchange, introducing HIC to improve robustness while enabling further optimization
